Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type III Secretion Using Split Fluorescent Protein Fragments.

Spatiotemporal Monitoring of Pseudomonas Effectors via Type III Secretion Using Split Fluorescent Protein Fragments

Pathogenic gram-negative bacteria cause serious diseases in animals and plants. These bacterial pathogens use the type III secretion system (T3SS) to deliver effector proteins into host cells; these effectors then localize to different subcellular compartments to attenuate immune responses by altering biological processes of the host cells. The fluorescent protein (FP)-based approach to monitor...

Peer Review Report for 10.1105/tpc.17.00047 Spatiotemporal Monitoring of Pseudomonas Effectors via Type III Secretion using Split Fluorescent Protein Fragments

Review timeline: TPC2017-00047-BRR Submission received: Jan. 16, 2017 1st Decision: Feb. 21, 2017 revision requested TPC2017-00047-BRR1 1st Revision received: April 24, 2017 2nd Decision: May 20, 2017 revision requested TPC2017-00047-BRR2 2nd Revision received: May 27, 2017 3rd Decision: June 7, 2017 acceptance pending, sent to science editor Final acceptance: June 14, 2017 Advance publication:...

Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin.

The bacterial plant pathogen Pseudomonas syringae requires a type III protein secretion system (TTSS) to cause disease. The P. syringae TTSS is encoded by the hrp-hrc gene cluster. One of the genes within this cluster, hrpJ, encodes a protein with weak similarity to YopN, a type III secreted protein from the animal pathogenic Yersinia species. Here, we show that HrpJ is secreted in culture and ...

Secretion of the HrpZ1 Harpin Pathogenesis, Injection of Effectors, and Secreted Protein That Is Required for Plant HrpJ Is a Type III Pseudomonas syringae

The majority of the type III effector inventory of Pseudomonas syringae pv. tomato DC3000 can suppress plant immunity.

The Pseudomonas syringae type III protein secretion system (T3SS) and the type III effectors it injects into plant cells are required for plant pathogenicity and the ability to elicit a hypersensitive response (HR). The HR is a programmed cell death that is associated with effector-triggered immunity (ETI). A primary function of P. syringae type III effectors appears to be the suppression of ET...